calculations on stock solutions?be sure to include the amount of water added and include all units 9. calculate how to use the 20% stock solution of sds to make a100 ml of a 0.1% sds working solution 10. calculate how to use the 0.5 m tris stock solutions to make the 350 ml of a 10 mm tris working solution. calculations on multiple stock solutions?be sure to include the amount of water added and include all units 11. calculate how to use the 1 m tris and 0.2 m edta stock solutions to make the 400 ml of a of 10 mm tris and 1 mm edta solution. 12. calculate how to use a 3 m nacl and 10% np40 stock solutions to make a 500 ml of a 300 mm nacl and a 0.2% np40 working solution.
C1V1 = C2V2
where,
C1 – concentration of the stock solution
V1 – volume of the stock solution required
C2 = concentration of the working solution
V2 = volume of the working solution
a) 20% x V1 = 0.1% x 100mL
V1 = 0.5ml
Therefore, 0.5mL of stock solution (20% SDS) needs to be added to 99.5mL water to make a working solution of 0.1% SDS.
b) 500mM x V1 = 10mM x 350mL
V1 = 7mL
Therefore, 7mL of stock solution (0.5M Tris) needs to be added to 343mL water to make a working solution of 10mM Tris.
c) 1000mM x V1 = 10mM x 400
V1 = 4mL 1M Tris
200mM x V1 = 1mM x 400
V1 = 2 mL 0.2M EDTA
Therefore, 4mL of stock solution (1M Tris) and 2mL of stock solution (0.2M EDTA) needs to be added to 394mL water to make a working solution of 10mM Tris and 1mM EDTA.
d) 3000mM x V1 = 300mM x 500mL
V1 = 50mL
10% x V1 = 0.2% x 500mL
V1 = 10mL
Therefore, 50mL of stock solution (3M NaCl) and 10mL of stock solution (10% NP40) needs to be added to 440mL water to make a working solution of 300mM NaCl and 0.2% NP40.