After boiling the leaf in ethanol why did you rinse the leaf in water

after boiling the leaf in ethanol why did you rinse the leaf in water

Why rinse the leaf in water after boiling it in ethanol?

Answer:
Rinsing the leaf in water after boiling it in ethanol serves an important purpose in laboratory experiments or scientific processes. When a leaf is boiled in ethanol, the ethanol dissolves the chlorophyll pigment, removing the green color from the leaf. However, ethanol can also dehydrate the leaf and make it brittle. Therefore, rinsing the leaf in water after boiling it in ethanol helps to rehydrate the leaf, making it more pliable for further experimentation. Additionally, rinsing the leaf in water helps to remove any excess ethanol residue, ensuring that the results of the experiment are accurate and not skewed by the presence of ethanol. This process allows for the leaf to be in a suitable condition for further analyses or observations without the interference of residual ethanol.