what is the difference between transcription and translation
What is the difference between transcription and translation?
Transcription:
Transcription is the process in which the genetic information encoded in DNA is copied into a complementary RNA sequence. This process occurs in the nucleus of eukaryotic cells or in the cytoplasm of prokaryotic cells. Here are the key points about transcription:
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Location: Transcription primarily occurs in the nucleus of eukaryotic cells, while in prokaryotic cells, it happens in the cytoplasm.
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Enzymes: RNA polymerase is the enzyme responsible for carrying out transcription. It binds to the DNA template and synthesizes a complementary RNA molecule.
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Template: During transcription, one strand of the DNA molecule serves as a template for the synthesis of an RNA molecule. This strand is called the template or antisense strand.
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Product: The end product of transcription is a single-stranded RNA molecule that is complementary to the DNA template. This RNA molecule is known as messenger RNA (mRNA) in protein-coding genes.
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Purpose: Transcription is a crucial step in gene expression, as it produces RNA molecules that serve as templates for protein synthesis during translation.
Translation:
Translation is the process in which the information carried by mRNA is decoded to produce a specific sequence of amino acids, forming a polypeptide chain or protein. Translation takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Here are the key points about translation:
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Location: Translation occurs in the cytoplasm, specifically on ribosomes, which are the cellular structures where protein synthesis takes place.
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Machinery: Translation requires several key components, including mRNA, transfer RNA (tRNA), ribosomes, and various protein factors.
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Codons: mRNA carries the genetic information in the form of a series of three-nucleotide sequences called codons. Each codon specifies a particular amino acid or a stop signal.
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tRNA and Amino Acids: During translation, tRNA molecules bring specific amino acids to the ribosome based on the codon sequence on the mRNA. The ribosome catalyzes the formation of peptide bonds between adjacent amino acids, resulting in the elongation of the polypeptide chain.
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Product: The end product of translation is a polypeptide chain or protein, depending on the sequence of amino acids specified by the mRNA.
In summary, transcription involves the synthesis of RNA from a DNA template, while translation involves the synthesis of proteins from an mRNA template. Transcription occurs in the nucleus of eukaryotic cells or in the cytoplasm of prokaryotic cells, whereas translation occurs in the cytoplasm on ribosomes. These processes are fundamental to gene expression and protein synthesis in living organisms.